Abstract

The records (1971-1985) of Dr. Russell F. Doolittle's biochemistry laboratory at the University of California, San Diego include notebooks related to the first determination of the complete sequence of amino acids in the human fibrinogen molecule, paper files for the amino acid sequences contained in the protein sequence data bank called NEWAT and an amino acid sequencing machine. The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3) AMINO ACID SEQUENCER.

Administrative History

Dr. Russell F. Doolittle, professor of chemistry at the University of California, San Diego, heads a campus science laboratory that conducts research in the evolutionary and structural aspects of proteins. In 1979, Doolittle's laboratory successfully analyzed the structure of the amino acid sequence for the human fibrinogen molecule.

During that study, ten to twenty postdoctoral, graduate and undergraduate researchers worked to pull apart and analyze the amino acid sequences in the alpha, beta and gamma chains of fibrinogen. Doolittle adapted a peptide synthesizing machine to break apart peptides and employed an amino acid analyzer to identify the sequences.

The peptide synthesizer, which was the second peptide synthesizer ever constructed, was modeled on the prototype designed by Bruce Merrifield, then of Rockefeller University. Merrifield was later awarded the Nobel Prize for the design of the machine.

The UCSD machine was constructed in 1964 at the instigation of Martin Kamen, a member of the Chemistry faculty. Professor Kamen had seen Merrifield's machine and, on his return to UCSD, asked Arthur Robinson, a graduate student in the Chemistry Department, to oversee construction of the machine. Robinson journeyed back to New York to examine Merrifield's machine more closely and then, after returning to UCSD, began constructing the UCSD machine.

After a period of time, the machine was converted into a peptide sequencer. Instead of building up peptides one amino acid at a time, it would take them apart one amino acid at a time. In 1979, the machine was used to make the first determination of the complete amino acid sequence for the human fibrinogen molecule, which is composed of 2,934 amino acids.

Doolittle also constructed a protein sequence data bank called NEWAT. Sequences identified in the published literature were entered into an electronic database and new sequences were searched against known sequences. In 1983, Doolittle used the data bank to link separate research efforts at the Center for Blood Research in Boston and the National Cancer Institute in Washington, D. C. He related a known cancer gene to a gene with a known function in human cells.

Scope and Content

The Russell Doolittle Biochemistry Laboratory Records document the activities of a UCSD science laboratory engaged in research on the structure of the human fibrinogen molecule. The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3) AMINO ACID SEQUENCER.

SERIES 1: NEWAT

The NEWAT series contains journal articles, printouts and summary data reports for individual amino acid sequences that were entered into the electronic data bank. The series begins with a list that relates the folder title acronym to the name and the journal citation for each amino acid sequence. The materials are arranged alphabetically by acronym.

SERIES 2: LABORATORY NOTEBOOKS

The LABORATORY NOTEBOOKS series contains notebooks by individual postdoctoral, graduate and undergraduate researchers who worked on the project. Also included are notebooks that contain amino acid analysis charts and high performance liquid chromotography charts. The notebooks are arranged by name of researcher, subproject and type of chart as follows: 2A) Watt, Ken; 2B) Bouma, H.; 2C) Takagi, T.; 2D) Miscellaneous Researchers; 2E) Electric Birefringence; 2F) Peptide Synthesis; 2G) Analysis Charts; and 2H) High Performance Liquid Chromotography Charts.

SERIES 3: AMINO ACID SEQUENCER

This is the machine adapted by laboratory staff to pull apart amino acids.

Laboratory notebooks.

Additions to the Russell Doolittle Biochemistry Laboratory Records.

BoxFolderOversize
11 Index to acronyms.
12 2GPH to ATHT.
21 ATPC1, -2, -3, -4, -5 to CHEY.
31 CHEZ to DEGC.
41 DGI7 to FBCH1.
51 F7BO to GYPD 1 and 2.
61 HICT to IF2B.
71 IF3E to LYMH.
81 M3RD to ORDN.
91 OREC to PSPB.
101 PSI1 to PYRP.
111 SIRV to TFWS.
121 TFT4 to VP2S.
131 VPIM to ZUKE.

LABORATORY NOTEBOOKS

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Watt, Ken

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141 Book 1 - Part 1. Beta Chain CNBr / Lysozyme HSer Coupling, 10/01/76 - 09/30/78.
142 Book 1 - Part 2. Beta Chain CNBr / Lysozyme HSer Coupling, 01/01/77 - 08/31/77.
143 Book 2 - Part 1. H Beta 0 CN I, 11/01/76 - 08/31/78.
144 Book 2 - Part 2. H Beta 0 CN I, 11/01/76 - 08/31/78.
145 Book 2 - Part 3. H Beta 0 CN I, 11/01/76 - 08/31/78.
146 Book 3. H Beta 0 CN V, 11/01/76 - 06/30/77.
147 Book 4. H Beta 0 CN VII, 11/01/76 - 07/31/77.
148 Book 5 - Part 1. H Beta 0 CN II, 11/01/76 - 09/30/78.
149 Book 5 - Part 2. H Beta 0 CN II, 11/01/76 - 09/30/78.
1410 Book 6 - Part 1. H Beta 0 CN VI, 11/01/76 - 12/31/77.
1411 Book 6 - Part 2. H Beta 0 CN VI, 11/01/76 - 12/31/77.
1412 Book 7. Sequence H Beta CN VId, 12/02/76 - 03/31/78.
1413 Book 8. H Beta CN II, Glu Cleavage, 01/01/77 - 11/30/77.
1414 Book 9. H Beta CN IV, 01/01/77 - 12/31/77.
151 Book 10. H Beta CN III, 01/01/77 - 08/31/77.
152 Book 11 - Part 1. Citraconylation, Triptic Digestion of Human 0 Beta Chain, 03/01/77 - 06/01/77.
153 Book 11 - Part 2. Citraconylation, Tryptic Digestion of Human 0 Beta Chain, 03/01/77 - 06/30/77.
154 Book 12. H Beta CN Vb, 03/01/77 - 08/31/77.
155 Book 13. CN VI C-2, CN VIf, H Beta CVI VI C-3, CN VI b-1, CN VIC-4, CN VI C-1, CN VIa, 03/01/77 - 05/31/77.
156 Book 14. H Beta CN IVb, 03/01/77 - 07/31/77.
157 Book 15 - Part 1. H Beta CN IIIa from Re-chromatog, 05/01/77 - 10/31/77.
158 Book 15 - Part 2. H Beta CN IIIa from Re-chromatog, 05/01/77 - 10/31/77.
159 Book 16. H Beta CN IIIb, CN IVa, 07/01/77 - 08/31/77.
1510 Book 17 - Part 1. H Beta CN V TR, 07/01/77 - 12/31/77.
1511 Book 17 - Part 2. H Beta CN V TR, 07/01/77 - 12/31/77.
1512 Book 18. H Beta CN IV TR, Glu, 07/01/77 - 09/31/77.
1513 Book 19 - Part 1. H Beta CN IVe, 07/01/77 - 10/31/77.
1514 Book 19 - Part 2. H Beta CN IVe, 07/01/77 - 10/31/77.
1515 Book 20 - Part 1. H Beta CN III CT and TR, 07/01/77 - 08/31/77.
1516 Book 20 - Part 2. H Beta CN III CT and TR, 07/01/77 - 08/31/77.
1517 Book 21. H Alpha 6d Ch, TR, 08/01/77 - 06/30/79.
1518 Book 22. H Beta CN V Glu-, 10/01/77 - 01/31/78.
161 Book 23. H Beta CN III TR, 10/1/77 - 10/31/77.
162 Book 24 - Part 1. H Beta CT, 11/01/77 - 01/31/78.
163 Book 24 - Part 2. H Beta CT, 11/01/77 - 03/31/78.
164 Book 25. H Beta CN Ne CT, and Sequence, 12/01/77 - 12/31/77.
165 Book 26 - Part 1. H Beta Glu, 12/01/77 - 2/28/78.
166 Book 26 - Part 2. H Beta Glu, 12/01/77 - 2/28/78.
167 Book 26 - Part 3. H Beta Glu, 12/01/77 - 2/28/78.
168 Book 27 - Part 1. H Beta CN II Glu EDC Sequence, 01/01/78 - 01/31/78.
169 Book 27 - Part 2. H Beta CN II Glu EDC Sequence, 01/01/78 - 01/31/78.
1610 Book 28 - Part 1. Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 - 05/31/78.
1611 Book 28 - Part 2. Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 - 05/31/78.
1612 Book 29. H Beta CNI GEE DITC Bends, 09/01/78 - 09/31/78.
1613 Book 30 - Part 1. H Alpha CN1 PL1 SP1 Ch, 10/01/78 - 03/31/79.
1614 Book 30 - Part 2. H Alpha CN1 PL1 SP1 Ch, 10/01/78 - 03/31/79.
1615 Book 31 - Part 1. H Alpha CNI SPI Ch 2nd, 11/01/78 - 02/28/79.
1616 Book 31 - Part 2. H Alpha CNI SPI Ch 2nd, 11/01/78 - 02/28/79.
1617 Book 32. PLI SP2 Ch to SGSFRP...E, ENI SP Pool 2, 01/01/79 - 02/28/79.
1618 Book 33. H Alpha CN1 SP2 Ch SP2dnp to Ng, 02/01/79 - 03/31/79.
1619 Book 34. H Alpha CN1 SP1 Ch, 02/01/79 - 03/31/79.
1620 Book 35 - Part 1. H Alpha Chain, 04/01/79 - 06/30/79.
171 Book 35 - Part 2. H Alpha Chain, 04/01/79 - 06/30/79.
172 Book 35 - Part 3. H Alpha Chain, 04/01/79 - 06/30/79.
173 Book 36 - Part 1. H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 - 05/31/79.
174 Book 36 - Part 2. H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 - 05/31/79.
175 Book 37 - Part 1. H Alpha Glu (sequence) 71mg prep, 05/01/79 - 06/30/79.
176 Book 37 - Part 2. H Alpha Glu (sequence) 71mg prep, 05/01/79 - 06/30/79.
177 Book 38 - Part 1. H Alpha - Subscript 2 - SP 72 6 mg G50, 06/01/79 - 06/30/79.
178 Book 38 - Part 2. H Alpha - Subscript 2 - SP 72 6mg G50, 06/01/79 - 06/30/79.
179 Book 38 - Part 3. H Alpha - Subscript 2 - SP 72 6mg G50, 06/01/79 - 06/30/79.
1710 Book 38 - Part 4. H Alpha - Subscript 2 - SP 72 6 mg G50, 06/01/79 - 06/30/79.
1711 Book 39. Ch H Alpha CNI PLII, 03/01/78 - 07/31/78.
1712 Book 40 - Part 1. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78.
1713 Book 40 - Part 2. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78.
1714 Book 40 - Part 3. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78.
1715 Book 41 - Part 1. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78.
1716 Book 41 - Part 2. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78.
1717 Book 41 - Part 3. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78.
1718 Book 42. H Alpha, CNI Tryptophan, 04/01/78 - 06/30/78.
1719 Book 43 - Part 1. H Beta Chain Trypson, 04/01/78 - 05/31/78.
1720 Book 43 - Part 2. H Beta Chain Trypson, 04/01/78 - 05/31/78.
1721 Book 44. Frag. D, CN III C, TR, 05/01/78 - 07/31/78.
181 Book 45 - Part 1. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78.
182 Book 45 - Part 2. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78.
183 Book 45 - Part 3. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78.
184 Book 46. H Alpha CNI PLI Ch, 06/01/78 - 06/30/78.
185 Book 47. H Alpha CNI PLI Ch (EDC), 04/01/78 - 08/31/78.
186 Book 48. H Alpha CNI PL2, Ch2, Ch1, 07/01/78 - 07/31/78.
187 Book 49. II H Alpha CNI Trytophan, 08/01/78 - 03/31/78.
188 Book 50 - Part 1. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ration, partially GEE, 08/01/78 - 01/31/79.
189 Book 50 - Part 2. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 - 01/31/79.
1810 Book 50 - Part 3. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 - 01/31/79.

Bouma, H.

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1811 Book 1 - Part 1. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76.
1812 Book 1 - Part 2. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76.
1813 Book 1 - Part 3. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76.
1814 Book 2 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76.
1815 Book 2 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76.
1816 Book 2 - Part 3. Human Fibrinogen; Gamma Chain; Citrconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76.
1817 Book 3. Miscellaneous: Anti-human Fibrinogen; 4, 4'-diaminobenzil; TATG synthesis, 09/01/75- 09/30/76.
1818 Book 4 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76.
191 Book 4 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76.
192 Book 4 - Part 3. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76.
193 Book 5. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks V and VI, 01/01/76 - 08/31/76.
194 Book 6 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin, G-25, 01/01/76 - 03/31/76.
195 Book 6 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin, G-25, 01/01/76 - 03/31/76.
196 Book 7 - Part 1. Human Fibrinogen; Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6, 10/01/76 - 01/31/77.
197 Book 7 - Part 2. Human Fibrinogen, Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6, 10/01/76 - 01/31/77.
198 Book 8 - Part 1. Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 - 08/31/77.
199 Book 8 - Part 2. Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 - 08/31/77.
1910 Book 9 - Part 1. Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications (aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 - 11/31/76.
1911 Book 9 - Part 2. Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications (aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 - 11/31/76.
1912 Book 10 - Part 1. HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77 - 04/30/77.
1913 Book 10 - Part 2. HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77 - 04/30/77.
1914 Book 11 - Part 1. HG, CT 3B; TRa-2; TRb-2, 04/01/77 - 06/30/77.
1915 Book 11 - Part 2. HG, CT 3B; TRa-2; TRb-2, 04/01/77 - 06/30/77.
1916 Book 12 - Part 1. Plasmin - 0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 - 01/31/78.
1917 Book 12 - Part 2. Plasmin - 0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 - 01/31/78.
1918 Book 13 - Part 1. HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 - 12/31/77.
201 Book 13 - Part 2. HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 - 12/31/77.
202 Book 14. HF, PLD, CN II; HF, PLD, CN III, 02/01/78 - 05/31/78.
203 Book 15 - Part 1. G - 37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 - 01/31/78.
204 Book 15 - Part 2. G - 37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 - 01/31/78.
205 Book 16 - Part 1. HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 - 03/31/78.
206 Book 16 - Part 2. HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 - 03/31/78.
207 Book 16 - Part 3. HF, PLD, CN I, TRA; HF, PHD, CN I, TRB, 09/01/77 - 11/30/77.
208 Book 16 - Part 4. HF, PLD, CN I, TRA; HF, PLD, CN I, TR B, 09/01/77 - 11/30/77.
209 Book 17 - Part 1. Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 - 06/30/78.
2010 Book 17 - Part 2. Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 - 06/30/78.
2011 Book 18. Tryptic-digest; Human Fibrinogen; Gamma Chain, 06/01/78 - 07/31/78.
2012 Book 19. Fragment D; CN I, TRA; pH 7.0 Trypsin; G-25, 04/01/78 - 07/31/78.

Takagi, T.

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2013 Book 1 - Part 1. H Gamma CT Pools I, II, III, DNS; CT I, II, III TATG (electrophoresis, CNBr), 11/01/73 - 07/31/74.
2014 Book 1 - Part 2. H Gamma CT Pools I, II, III, DNS; CT I, II, III, TATG (electrophoresis, CNBr), 11/01/73 - 07/31/74.
2015 Book 2 - Part 1. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74.
2016 Book 2 - Part 2. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74.
2017 Book 2 - Part 3. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74.
2018 Book 3 - Part 1. H Gamma CNB R and A; Trypsin I - IV (gel filtration, paper el.), 05/01/74 - 08/31/74.
211 Book 3 - Part 2. H Gamma CNB R and A; Trypsin I - IV (gel filtration, paper el.), 05/01/74 - 08/31/74.
212 Book 4. H Gamma chain; AAA 24, 48,72 hr.; Tryptophan Det with Methane Sulfonic Acid; H Gamma CNI AAA 3/75, 07,01/73 - 12/31/73.
213 Book 5 - Part 1. H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 - 11/30/74.
214 Book 5 - Part 2. H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 - 11/30/74.
215 Book 6. H Gamma CNI Pepsin Gel Filtration G-25 Paper El, 10/01/74 - 11/30/74.
216 Book 7 - Part 1. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74.
217 Book 7 - Part 2. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74.
218 Book 7 - Part 3. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74.
219 Book 8. Gamma CN III, IV, V, 09/01/73 - 10/31/74.
2110 Book 9 - Part 1, 1 of 2. 11/73.
2111 Book 9 - Part 1, 2 of 2. 11/73.
2112 Book 9 - Part 2, 1 of 2. 11/73.
2113 Book 9 - Part 2, 2 of 2. 11/73.
2114 Book 10, Collagenase Digestion of H0. 3/74 - 4/74.
2115 Book 11 - Part 1, 1 of 2 -- Plasmin Digestion of. 4/74 - 5/74.
2116 Book 11 - Part 1, 2 of 2 -- Plasmin Digestion of. 4/74 - 5/74.
2117 Book 11 - Part 2, 1 of 2 -- Plasmin Digestion of H0 90' 21hr. 4/74 - 5/74.
2118 Book 11 - Part 2, 2 of 2 -- Plasmin Digestion of H0 90' 21 hr. 4/74 - 5/74.
2119 Book 12 - Part 1 -- HD, CNBr G-100 Purification, Enzyme Digestions. 1/75 - 3/75.
221 Book 12 - Part 2. 01/75 - 03/75.
222 Book 13. Separation of D and E on Biogel A 1.5 M, 4 m Guanidine, 02/74 - 07/74.
223 Book 14. HDII: Separation, R and A, Purification, Enzyme Digestion, 08/74 - 12/74.
224 Book 15 - Part 1. HE R and A -- G-25, Paper El, 07/74.
225 Book 15 - Part 2. 07/74.
226 Book 16 - Part 1. Gel Filtration of D and E, HE CNBr -- G-50, Paper El of Peptides, 05/74- 07/74.
227 Book 16 - Part 2. 05/74 - 07/74.
228 Book 17 - Part 1. HE CNBr (S-S) in E, Purification of CNBr Peptides, 08/74 - 01/75.
229 Book 17 - Part 2. 08/74 - 01/75.
2210 Book 18 - Part 1. FIIB-2, FIIB-3, FIIB-4, FIIB-5, D-D3, 02/74 - 02/75.
2211 Book 18 - Part 2. 02/74 - 01/75.
2212 Book 19 - Part 1. CNBr Digestion of H Fibrin, 10/74 - 02/75.
2213 Book 19 - Part 2. 10/74 - 02/75.
2214 Book 20 - Part 1. TATG of H Fragments D, E, 02/74.
2215 Book 20 - Part 2. TATG of H Fragment D, E, 02/74.
2216 Book 21. H0 CNBR, H Gamma CNBr, 02/74 - 5/74.
2217 Book 22. H 14C GEE Labelled Gamma Chain, Fragments from Plasmin Digestion of H GEE Fibrins, 02/74 - 05/74.
2218 Book 23. TATG of H Fragment D, E (see envelope), 05/74 - 8/74.
2219 Book 24. Human Gamma G-50, Purfication of Pools 4.5 and 6, Enzyme Digestion of Cyanogen Bromide Pools, 12/74 - 02/75.
231 Book 25 - Part 1. Beta chain CNBr, G-100 10% HAc Purfication, 08/74 - 12/75.
232 Book 25 - Part 2. 08/75 - 12/75.
233 Book 26 - Part 1. Separation of H Gamma, Beta Chains CM52, Beta Chain CNBr Peptides AAA, 03/74.
234 Book 26 - Part 2. 03/74.
235 Book 27. Bov Gamma CNBr A,B,C, AAA (Bovine).
236 Book 28. Oxidized Bov Gamma Chain and Its CNBr Fragments, Separation Biogel 1.5 Purification by Paper El, 01/74 - 02/74.
237 Book 29. H Fragment A from Plasmin, digest 10', 90', 15 hrs, Enzyme Digestion, 05/72.
238 Book A - Part 1. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platelet "X", 12/01/72 - 10/31/73.
239 Book A - Part 2. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X", 12/01/72 - 10/31/73.
2310 Book A - Part 3. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X".
2311 Book A - Part 4. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X", 12/01/72 - 10/31/73.
2312 Book B - Part 1. Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels); Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *; Separation of A and B Chains of F XIII *, 09/01/72 - 09/30/73.
2313 Book B - Part 2. Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels); Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *; Separation of A and B Chains of F XIII *, 09/01/72 - 09/30/73.
2314 Book C - Part 1. TATG: H, B FXIII, FXIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72 - 11/31/73.
2315 Book C - Part 2. TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII; Seattle F XIII *, 11/01/72 - 11/31/73.
2316 Book C - Part 3. TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72 - 11/31/73.
2317 Book D - Part 1. Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th, 10/01/72 - 10/31/73.
2318 Book D - Part 2. Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th, 10/01/72 - 10/31/73.
2319 Book E. Bov Gamma ( plasmin, trypsin SDS gels ); GEE B Gamma - Plasmin; B 0 plasmin, 08/01/72 - 03/31/73.

Miscellaneous Researchers

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241 Friezner, S - Book 1 - Part 1. H Gamma CNI Trypsin, 06/75.
242 Friezner, S - Book 1 - Part 2. 06/75.
243 Lin, Y - Book 1. H 14C GEE Alpha Electrophoresis; T-boc Reactions T-boc-EA, 02/01/71 - 05/31/71.
244 Lin, Y - Book 2 - Part 1. Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains, 02/01/71 - 05/31/74.
245 Lin, Y - Book 2 - Part 2. Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains, 02/01/71 - 05/31/74.
246 Maffly, B. and Goldbaum, D - HPLC. 04/76 - 01/78.

Electric Birefringence

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247 Cottrell, B. - Book 1 - Part 1, 8/73 - 11/73.
248 Cottrell, B. - Book 1 - Part 2, 8/73 - 11/73.
249 Cottrell, B. - Book 2 - Part 1, 1973. Photographs, computer programs and basic user manual.
2410 Cottrell, B. - Book 2 - Part 2, 4/74 - 6/74.
2411 Cottrell, B. - Book 2 - Part 3, 4/74 - 6/74.
2412 Cottrell, B. - Book 3 - Part 1, 6/74 - 7/74.
2413 Cottrell, B. - Book 3 - Part 2, 6/74 - 7/74.
2414 Cottrell, B. - Book 4 - Part 1, 8/74 - 3/75.
2415 Cottrell, B. - Book 4 - Part 2, 8/74 - 7/75.
2416 Cottrell, B. - Book 5 - Part 1, 3/75 - 5/75.
2417 Cottrell, B. - Book 5 - Part 2, 3/75 - 5/75.
2418 Cottrell, B. - Computer Graphs B/C.
251 Cottrell, B. - Computation Book, Oct 22, 1973.
252 Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 1.
253 Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 2.
254 Cottrell, B. - Data Output. Elbra - Part 1.
255 Cottrell, B. - Data Output. Elbra - Part 2.
256 Cottrell, B. - Log Book. Photographs.
257 Cottrell, B. - Log Book. Photographs - Enlarged (part II).
258 Troll, M. - Book 1. System logic of the laser birefringence machine, reprints, technical information, equipment specifications (part 1).
259 Troll, M. - Book 1. System logic of laser birefringence machine, reprints, technical information, equipment specifications (part II).

Peptide Synthesis

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2510 BM BIOCHEMICA - Vol. 2 Number 3, 1985.
2511 Book 9.
2512 Book 10.
2513 Computation Notebook #1.
2514 Computation Notebook #2.
2515 Computation Notebook #3.
2516 Computation Notebook #4.
2517 Computation Notebook #5.
2518 Computation Notebook #6.
261 Computation Notebook #7.
262 Computation Notebook #8.
263 Computation Notebook #9.
264 Computation Notebook #10.
265 Computation Notebook #11.
266 Computation Notebook #12.
267 Deoxynucleotide Synthesis, Phosphonylations, Monomer Synthesis, Dimer Synthesis.
268 DNA Sequencing 3:728-3D, 1985.
269 D.T. Peptide Synthesis.
2610 Evans, Barbara - Untitled notebook.
2611 Gel Sequencing.
2612 Helices.
2613 Lab Reports.
2614 Lamprey Transferrin (Part I). Barbara Evans, Summer 1983.
2615 Lamprey Transferrin (Part II). Barbara Evans, Summer 1983.
2616 Lamprey Transferrin: DNA Sequencing 463-1B, 1985 - 1986. Barbara Evans.
2617 Lamprey Transferrin: Plasmid Preps, 1984. Barbara Evans.
2618 Lamprey Transferrin: Sequence 1 Molecular Biology, 1984 - 1985. Barbara Evans.
2619 Lamprey Transferrin: Sequencing 2: 613-3B, 463-1B, 1985. Barbara Evans.
2620 Nucleotide Synthesis - Ologonucleotide Probes, 1982. Barbara Evans.
2621 Peptide Synthesis - James Kobut/R. Doolittle.
271 Pontes, Manuel.
272 Pontes, Manuel.
273 RD and MTNICB0.

Analysis Charts

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274 2 VB Sequence.
275 AAA of Gamma Chains Trp Det.
276 Bead Data SP Digest of PLI (Part I).
277 Bead Data SP Digest of PLI (Part II).
278 Bead Data SP Digest PLI (Part II continues).
279 Bov Gamma Chains, 1973.
2710 Bov Gamma chains II.
2711 CN I, PL I, SP I, PL II, Tryptic Data, Chymo, SP Peptides, 1979.
2712 CN - V B, C, and D. B Gamma CN from pH2, SC - pH 9.5, CN5B - pH 9.5, SD - pH 4.5.
2713 CNBr, Cyanogen Bremide G-75 Runs.
2714 Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part I).
2715 Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part II).
2716 D1 Binder D1 Non Binder 12/80 R and A Trypsin.
281 D2 Alpha - Trypsin.
282 D2 Rho Glass Beads.
283 H Alpha 2, CNI, TFA, CN IB, Trypsin, part 1.
284 H Alpha 2, CNI, TFA, CN IB, Trypsin, part 2.
285 H2 CN I PL I Se #2, S rho I TR - G-25, part 1.
286 H2 CN I PL I Se #2, S rho I TR - G-25, part 2.
287 H Alpha CNI CNBr = 70% TFA, H0CNI Pl I Glu, Pl 2 Glu.
288 H and CNI PL 3.
289 H Alpha 2, 1977.
2810 H Alpha 2, 1977.
2811 H Alpha CN, 1976.
2812 H Alpha CN III, 1976.
2813 H Alpha CN III, 1978.
2814 H Alpha CN III, 1978.
2815 H Alpha CN IV, 1975.
2816 H Alpha CN IV , 1975.
2817 H Alpha CN4A.
2818 H Alpha CN IV B.
291 H Alpha CN31.
292 H Alpha CN5B.
293 H Gamma Chain CN IV, 1975.
294 H Alpha CN CN -VII, 1977 (1 of 2).
295 H Gamma Chain CN V-VII, 1977 (2 of 2).
296 HD R and A BC 1980 D1 Gamma D3 Gamma Trypsin.
297 H Gamma chain, 1971.
298 H Gamma chain, 1972.
299 H Gamma chain, 1972.
2910 Gamma Chains Preps CN III, CNIV.
2911 HXLF CEE 7177 CN I Tal 23.
2912 Machine H Alpha CN III "G7B", 1976.
2913 Mellitin Sequence Run, 07/18/80.
2914 Sequence Guide 1969-70.
2915 Sequenator log sheet.
301 SP Digest #4 PLI, TR Digest Over G.50, part 1.
302 SP Digest #4 PLI, TR Digest Over G.50, part 2.
303 SP Digest CNI #6, SP Digest CNI #9 (8/79), SP Digest CNI #11 (4/79).
304 SP of PLI #3, SP of PLI #5. 11/15/78.
305 Trypsin Digest.
306 Untitled.
307 Untitled.
308 Untitled.
309 Untitled.
3010 Untitled.
3011 Untitled.
3012 Untitled.
3013 Untitled.
3014 Untitled.
3015 Untitled.

High Performance Liquid Chromotography Charts

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311 Untitled.
312 Untitled.
313 Untitled.
314 Acylase, N-Ac-Ab-C-Sar.
315 CMC.
316 Electrofocusing.
317 Funatsu Peptide.
318 HPLC Anion Exchange.
319 HPLC, DMG, PTH, STDS.
3110 HPLC JD.
3111 HPLC Reverse Phase, March-April 1976.

AMINO ACID SEQUENCER

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3112AB-000-01Amino Acid Sequencer.



Finding aid generated: 2005-10-28