The Register of
Russell Doolittle Biochemistry Laboratory Records
1971 - 1985
MSS 0077
Mandeville Special Collections Library
Geisel Library
University of California, San Diego
Extent: 33.00 linear feet (29 records cartons, 3 archives boxes and 1 art bin item)
Abstract
The records (1971-1985) of Dr. Russell F. Doolittle's biochemistry laboratory at the University of California, San Diego include notebooks related to the first determination of the complete sequence of amino acids in the human fibrinogen molecule, paper files for the amino acid sequences contained in the protein sequence data bank called NEWAT and an amino acid sequencing machine. The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3) AMINO ACID SEQUENCER.
Administrative History
Dr. Russell F. Doolittle, professor of chemistry at the University of California, San Diego, heads a campus science laboratory that conducts research in the evolutionary and structural aspects of proteins. In 1979, Doolittle's laboratory successfully analyzed the structure of the amino acid sequence for the human fibrinogen molecule.
During that study, ten to twenty postdoctoral, graduate and undergraduate researchers worked to pull apart and analyze the amino acid sequences in the alpha, beta and gamma chains of fibrinogen. Doolittle adapted a peptide synthesizing machine to break apart peptides and employed an amino acid analyzer to identify the sequences.
The peptide synthesizer, which was the second peptide synthesizer ever constructed, was modeled on the prototype designed by Bruce Merrifield, then of Rockefeller University. Merrifield was later awarded the Nobel Prize for the design of the machine.
The UCSD machine was constructed in 1964 at the instigation of Martin Kamen, a member of the Chemistry faculty. Professor Kamen had seen Merrifield's machine and, on his return to UCSD, asked Arthur Robinson, a graduate student in the Chemistry Department, to oversee construction of the machine. Robinson journeyed back to New York to examine Merrifield's machine more closely and then, after returning to UCSD, began constructing the UCSD machine.
After a period of time, the machine was converted into a peptide sequencer. Instead of building up peptides one amino acid at a time, it would take them apart one amino acid at a time. In 1979, the machine was used to make the first determination of the complete amino acid sequence for the human fibrinogen molecule, which is composed of 2,934 amino acids.
Doolittle also constructed a protein sequence data bank called NEWAT. Sequences identified in the published literature were entered into an electronic database and new sequences were searched against known sequences. In 1983, Doolittle used the data bank to link separate research efforts at the Center for Blood Research in Boston and the National Cancer Institute in Washington, D. C. He related a known cancer gene to a gene with a known function in human cells.
Scope and Content
The Russell Doolittle Biochemistry Laboratory Records document the activities of a UCSD science laboratory engaged in research on the structure of the human fibrinogen molecule. The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3) AMINO ACID SEQUENCER.
SERIES 1: NEWAT
The NEWAT series contains journal articles, printouts and summary data reports for individual amino acid sequences that were entered into the electronic data bank. The series begins with a list that relates the folder title acronym to the name and the journal citation for each amino acid sequence. The materials are arranged alphabetically by acronym.
SERIES 2: LABORATORY NOTEBOOKS
The LABORATORY NOTEBOOKS series contains notebooks by individual postdoctoral, graduate and undergraduate researchers who worked on the project. Also included are notebooks that contain amino acid analysis charts and high performance liquid chromotography charts. The notebooks are arranged by name of researcher, subproject and type of chart as follows: 2A) Watt, Ken; 2B) Bouma, H.; 2C) Takagi, T.; 2D) Miscellaneous Researchers; 2E) Electric Birefringence; 2F) Peptide Synthesis; 2G) Analysis Charts; and 2H) High Performance Liquid Chromotography Charts.
SERIES 3: AMINO ACID SEQUENCER
This is the machine adapted by laboratory staff to pull apart amino acids.
Laboratory notebooks.
Additions to the Russell Doolittle Biochemistry Laboratory Records.
NEWAT
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| 1 | 1 | Index to acronyms. | |
| 1 | 2 | 2GPH to ATHT. | |
| 2 | 1 | ATPC1, -2, -3, -4, -5 to CHEY. | |
| 3 | 1 | CHEZ to DEGC. | |
| 4 | 1 | DGI7 to FBCH1. | |
| 5 | 1 | F7BO to GYPD 1 and 2. | |
| 6 | 1 | HICT to IF2B. | |
| 7 | 1 | IF3E to LYMH. | |
| 8 | 1 | M3RD to ORDN. | |
| 9 | 1 | OREC to PSPB. | |
| 10 | 1 | PSI1 to PYRP. | |
| 11 | 1 | SIRV to TFWS. | |
| 12 | 1 | TFT4 to VP2S. | |
| 13 | 1 | VPIM to ZUKE. |
LABORATORY NOTEBOOKS
Return to MenuWatt, Ken
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| 14 | 1 | Book 1 - Part 1. Beta Chain CNBr / Lysozyme HSer Coupling, 10/01/76 - 09/30/78. | |
| 14 | 2 | Book 1 - Part 2. Beta Chain CNBr / Lysozyme HSer Coupling, 01/01/77 - 08/31/77. | |
| 14 | 3 | Book 2 - Part 1. H Beta 0 CN I, 11/01/76 - 08/31/78. | |
| 14 | 4 | Book 2 - Part 2. H Beta 0 CN I, 11/01/76 - 08/31/78. | |
| 14 | 5 | Book 2 - Part 3. H Beta 0 CN I, 11/01/76 - 08/31/78. | |
| 14 | 6 | Book 3. H Beta 0 CN V, 11/01/76 - 06/30/77. | |
| 14 | 7 | Book 4. H Beta 0 CN VII, 11/01/76 - 07/31/77. | |
| 14 | 8 | Book 5 - Part 1. H Beta 0 CN II, 11/01/76 - 09/30/78. | |
| 14 | 9 | Book 5 - Part 2. H Beta 0 CN II, 11/01/76 - 09/30/78. | |
| 14 | 10 | Book 6 - Part 1. H Beta 0 CN VI, 11/01/76 - 12/31/77. | |
| 14 | 11 | Book 6 - Part 2. H Beta 0 CN VI, 11/01/76 - 12/31/77. | |
| 14 | 12 | Book 7. Sequence H Beta CN VId, 12/02/76 - 03/31/78. | |
| 14 | 13 | Book 8. H Beta CN II, Glu Cleavage, 01/01/77 - 11/30/77. | |
| 14 | 14 | Book 9. H Beta CN IV, 01/01/77 - 12/31/77. | |
| 15 | 1 | Book 10. H Beta CN III, 01/01/77 - 08/31/77. | |
| 15 | 2 | Book 11 - Part 1. Citraconylation, Triptic Digestion of Human 0 Beta Chain, 03/01/77 - 06/01/77. | |
| 15 | 3 | Book 11 - Part 2. Citraconylation, Tryptic Digestion of Human 0 Beta Chain, 03/01/77 - 06/30/77. | |
| 15 | 4 | Book 12. H Beta CN Vb, 03/01/77 - 08/31/77. | |
| 15 | 5 | Book 13. CN VI C-2, CN VIf, H Beta CVI VI C-3, CN VI b-1, CN VIC-4, CN VI C-1, CN VIa, 03/01/77 - 05/31/77. | |
| 15 | 6 | Book 14. H Beta CN IVb, 03/01/77 - 07/31/77. | |
| 15 | 7 | Book 15 - Part 1. H Beta CN IIIa from Re-chromatog, 05/01/77 - 10/31/77. | |
| 15 | 8 | Book 15 - Part 2. H Beta CN IIIa from Re-chromatog, 05/01/77 - 10/31/77. | |
| 15 | 9 | Book 16. H Beta CN IIIb, CN IVa, 07/01/77 - 08/31/77. | |
| 15 | 10 | Book 17 - Part 1. H Beta CN V TR, 07/01/77 - 12/31/77. | |
| 15 | 11 | Book 17 - Part 2. H Beta CN V TR, 07/01/77 - 12/31/77. | |
| 15 | 12 | Book 18. H Beta CN IV TR, Glu, 07/01/77 - 09/31/77. | |
| 15 | 13 | Book 19 - Part 1. H Beta CN IVe, 07/01/77 - 10/31/77. | |
| 15 | 14 | Book 19 - Part 2. H Beta CN IVe, 07/01/77 - 10/31/77. | |
| 15 | 15 | Book 20 - Part 1. H Beta CN III CT and TR, 07/01/77 - 08/31/77. | |
| 15 | 16 | Book 20 - Part 2. H Beta CN III CT and TR, 07/01/77 - 08/31/77. | |
| 15 | 17 | Book 21. H Alpha 6d Ch, TR, 08/01/77 - 06/30/79. | |
| 15 | 18 | Book 22. H Beta CN V Glu-, 10/01/77 - 01/31/78. | |
| 16 | 1 | Book 23. H Beta CN III TR, 10/1/77 - 10/31/77. | |
| 16 | 2 | Book 24 - Part 1. H Beta CT, 11/01/77 - 01/31/78. | |
| 16 | 3 | Book 24 - Part 2. H Beta CT, 11/01/77 - 03/31/78. | |
| 16 | 4 | Book 25. H Beta CN Ne CT, and Sequence, 12/01/77 - 12/31/77. | |
| 16 | 5 | Book 26 - Part 1. H Beta Glu, 12/01/77 - 2/28/78. | |
| 16 | 6 | Book 26 - Part 2. H Beta Glu, 12/01/77 - 2/28/78. | |
| 16 | 7 | Book 26 - Part 3. H Beta Glu, 12/01/77 - 2/28/78. | |
| 16 | 8 | Book 27 - Part 1. H Beta CN II Glu EDC Sequence, 01/01/78 - 01/31/78. | |
| 16 | 9 | Book 27 - Part 2. H Beta CN II Glu EDC Sequence, 01/01/78 - 01/31/78. | |
| 16 | 10 | Book 28 - Part 1. Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 - 05/31/78. | |
| 16 | 11 | Book 28 - Part 2. Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 - 05/31/78. | |
| 16 | 12 | Book 29. H Beta CNI GEE DITC Bends, 09/01/78 - 09/31/78. | |
| 16 | 13 | Book 30 - Part 1. H Alpha CN1 PL1 SP1 Ch, 10/01/78 - 03/31/79. | |
| 16 | 14 | Book 30 - Part 2. H Alpha CN1 PL1 SP1 Ch, 10/01/78 - 03/31/79. | |
| 16 | 15 | Book 31 - Part 1. H Alpha CNI SPI Ch 2nd, 11/01/78 - 02/28/79. | |
| 16 | 16 | Book 31 - Part 2. H Alpha CNI SPI Ch 2nd, 11/01/78 - 02/28/79. | |
| 16 | 17 | Book 32. PLI SP2 Ch to SGSFRP...E, ENI SP Pool 2, 01/01/79 - 02/28/79. | |
| 16 | 18 | Book 33. H Alpha CN1 SP2 Ch SP2dnp to Ng, 02/01/79 - 03/31/79. | |
| 16 | 19 | Book 34. H Alpha CN1 SP1 Ch, 02/01/79 - 03/31/79. | |
| 16 | 20 | Book 35 - Part 1. H Alpha Chain, 04/01/79 - 06/30/79. | |
| 17 | 1 | Book 35 - Part 2. H Alpha Chain, 04/01/79 - 06/30/79. | |
| 17 | 2 | Book 35 - Part 3. H Alpha Chain, 04/01/79 - 06/30/79. | |
| 17 | 3 | Book 36 - Part 1. H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 - 05/31/79. | |
| 17 | 4 | Book 36 - Part 2. H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 - 05/31/79. | |
| 17 | 5 | Book 37 - Part 1. H Alpha Glu (sequence) 71mg prep, 05/01/79 - 06/30/79. | |
| 17 | 6 | Book 37 - Part 2. H Alpha Glu (sequence) 71mg prep, 05/01/79 - 06/30/79. | |
| 17 | 7 | Book 38 - Part 1. H Alpha - Subscript 2 - SP 72 6 mg G50, 06/01/79 - 06/30/79. | |
| 17 | 8 | Book 38 - Part 2. H Alpha - Subscript 2 - SP 72 6mg G50, 06/01/79 - 06/30/79. | |
| 17 | 9 | Book 38 - Part 3. H Alpha - Subscript 2 - SP 72 6mg G50, 06/01/79 - 06/30/79. | |
| 17 | 10 | Book 38 - Part 4. H Alpha - Subscript 2 - SP 72 6 mg G50, 06/01/79 - 06/30/79. | |
| 17 | 11 | Book 39. Ch H Alpha CNI PLII, 03/01/78 - 07/31/78. | |
| 17 | 12 | Book 40 - Part 1. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78. | |
| 17 | 13 | Book 40 - Part 2. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78. | |
| 17 | 14 | Book 40 - Part 3. H Alpha - Subscript 2 - CNI Ch-, 03/01/78 - 03/31/78. | |
| 17 | 15 | Book 41 - Part 1. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78. | |
| 17 | 16 | Book 41 - Part 2. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78. | |
| 17 | 17 | Book 41 - Part 3. H Alpha CNI Ch Sequence, 04/01/78 - 08/31/78. | |
| 17 | 18 | Book 42. H Alpha, CNI Tryptophan, 04/01/78 - 06/30/78. | |
| 17 | 19 | Book 43 - Part 1. H Beta Chain Trypson, 04/01/78 - 05/31/78. | |
| 17 | 20 | Book 43 - Part 2. H Beta Chain Trypson, 04/01/78 - 05/31/78. | |
| 17 | 21 | Book 44. Frag. D, CN III C, TR, 05/01/78 - 07/31/78. | |
| 18 | 1 | Book 45 - Part 1. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78. | |
| 18 | 2 | Book 45 - Part 2. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78. | |
| 18 | 3 | Book 45 - Part 3. H Beta (CN Br), HD Beta Tryptic, 05/01/78 - 09/30/78. | |
| 18 | 4 | Book 46. H Alpha CNI PLI Ch, 06/01/78 - 06/30/78. | |
| 18 | 5 | Book 47. H Alpha CNI PLI Ch (EDC), 04/01/78 - 08/31/78. | |
| 18 | 6 | Book 48. H Alpha CNI PL2, Ch2, Ch1, 07/01/78 - 07/31/78. | |
| 18 | 7 | Book 49. II H Alpha CNI Trytophan, 08/01/78 - 03/31/78. | |
| 18 | 8 | Book 50 - Part 1. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ration, partially GEE, 08/01/78 - 01/31/79. | |
| 18 | 9 | Book 50 - Part 2. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 - 01/31/79. | |
| 18 | 10 | Book 50 - Part 3. H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 - 01/31/79. |
Bouma, H.
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| 18 | 11 | Book 1 - Part 1. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76. | |
| 18 | 12 | Book 1 - Part 2. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76. | |
| 18 | 13 | Book 1 - Part 3. I. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks V, VI, VII, VIII, 09/01/75 - 03/31/76. | |
| 18 | 14 | Book 2 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76. | |
| 18 | 15 | Book 2 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76. | |
| 18 | 16 | Book 2 - Part 3. Human Fibrinogen; Gamma Chain; Citrconyl - Trypsin, G-50; Peaks II, III, IV, 09/01/75 - 12/31/76. | |
| 18 | 17 | Book 3. Miscellaneous: Anti-human Fibrinogen; 4, 4'-diaminobenzil; TATG synthesis, 09/01/75- 09/30/76. | |
| 18 | 18 | Book 4 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl - Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76. | |
| 19 | 1 | Book 4 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76. | |
| 19 | 2 | Book 4 - Part 3. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76 - 06/30/76. | |
| 19 | 3 | Book 5. Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks V and VI, 01/01/76 - 08/31/76. | |
| 19 | 4 | Book 6 - Part 1. Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin, G-25, 01/01/76 - 03/31/76. | |
| 19 | 5 | Book 6 - Part 2. Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin, G-25, 01/01/76 - 03/31/76. | |
| 19 | 6 | Book 7 - Part 1. Human Fibrinogen; Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6, 10/01/76 - 01/31/77. | |
| 19 | 7 | Book 7 - Part 2. Human Fibrinogen, Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6, 10/01/76 - 01/31/77. | |
| 19 | 8 | Book 8 - Part 1. Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 - 08/31/77. | |
| 19 | 9 | Book 8 - Part 2. Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1, 4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 - 08/31/77. | |
| 19 | 10 | Book 9 - Part 1. Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications (aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 - 11/31/76. | |
| 19 | 11 | Book 9 - Part 2. Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications (aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 - 11/31/76. | |
| 19 | 12 | Book 10 - Part 1. HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77 - 04/30/77. | |
| 19 | 13 | Book 10 - Part 2. HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77 - 04/30/77. | |
| 19 | 14 | Book 11 - Part 1. HG, CT 3B; TRa-2; TRb-2, 04/01/77 - 06/30/77. | |
| 19 | 15 | Book 11 - Part 2. HG, CT 3B; TRa-2; TRb-2, 04/01/77 - 06/30/77. | |
| 19 | 16 | Book 12 - Part 1. Plasmin - 0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 - 01/31/78. | |
| 19 | 17 | Book 12 - Part 2. Plasmin - 0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 - 01/31/78. | |
| 19 | 18 | Book 13 - Part 1. HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 - 12/31/77. | |
| 20 | 1 | Book 13 - Part 2. HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 - 12/31/77. | |
| 20 | 2 | Book 14. HF, PLD, CN II; HF, PLD, CN III, 02/01/78 - 05/31/78. | |
| 20 | 3 | Book 15 - Part 1. G - 37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 - 01/31/78. | |
| 20 | 4 | Book 15 - Part 2. G - 37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 - 01/31/78. | |
| 20 | 5 | Book 16 - Part 1. HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 - 03/31/78. | |
| 20 | 6 | Book 16 - Part 2. HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 - 03/31/78. | |
| 20 | 7 | Book 16 - Part 3. HF, PLD, CN I, TRA; HF, PHD, CN I, TRB, 09/01/77 - 11/30/77. | |
| 20 | 8 | Book 16 - Part 4. HF, PLD, CN I, TRA; HF, PLD, CN I, TR B, 09/01/77 - 11/30/77. | |
| 20 | 9 | Book 17 - Part 1. Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 - 06/30/78. | |
| 20 | 10 | Book 17 - Part 2. Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 - 06/30/78. | |
| 20 | 11 | Book 18. Tryptic-digest; Human Fibrinogen; Gamma Chain, 06/01/78 - 07/31/78. | |
| 20 | 12 | Book 19. Fragment D; CN I, TRA; pH 7.0 Trypsin; G-25, 04/01/78 - 07/31/78. |
Takagi, T.
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| 20 | 13 | Book 1 - Part 1. H Gamma CT Pools I, II, III, DNS; CT I, II, III TATG (electrophoresis, CNBr), 11/01/73 - 07/31/74. | |
| 20 | 14 | Book 1 - Part 2. H Gamma CT Pools I, II, III, DNS; CT I, II, III, TATG (electrophoresis, CNBr), 11/01/73 - 07/31/74. | |
| 20 | 15 | Book 2 - Part 1. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74. | |
| 20 | 16 | Book 2 - Part 2. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74. | |
| 20 | 17 | Book 2 - Part 3. H Gamma CT IV - VIII (gel filtration, paper el., ion exchange - Aminex A5), 11/01/73 - 07/31/74. | |
| 20 | 18 | Book 3 - Part 1. H Gamma CNB R and A; Trypsin I - IV (gel filtration, paper el.), 05/01/74 - 08/31/74. | |
| 21 | 1 | Book 3 - Part 2. H Gamma CNB R and A; Trypsin I - IV (gel filtration, paper el.), 05/01/74 - 08/31/74. | |
| 21 | 2 | Book 4. H Gamma chain; AAA 24, 48,72 hr.; Tryptophan Det with Methane Sulfonic Acid; H Gamma CNI AAA 3/75, 07,01/73 - 12/31/73. | |
| 21 | 3 | Book 5 - Part 1. H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 - 11/30/74. | |
| 21 | 4 | Book 5 - Part 2. H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 - 11/30/74. | |
| 21 | 5 | Book 6. H Gamma CNI Pepsin Gel Filtration G-25 Paper El, 10/01/74 - 11/30/74. | |
| 21 | 6 | Book 7 - Part 1. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74. | |
| 21 | 7 | Book 7 - Part 2. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74. | |
| 21 | 8 | Book 7 - Part 3. H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion, 06/01/73 - 02/28/74. | |
| 21 | 9 | Book 8. Gamma CN III, IV, V, 09/01/73 - 10/31/74. | |
| 21 | 10 | Book 9 - Part 1, 1 of 2. 11/73. | |
| 21 | 11 | Book 9 - Part 1, 2 of 2. 11/73. | |
| 21 | 12 | Book 9 - Part 2, 1 of 2. 11/73. | |
| 21 | 13 | Book 9 - Part 2, 2 of 2. 11/73. | |
| 21 | 14 | Book 10, Collagenase Digestion of H0. 3/74 - 4/74. | |
| 21 | 15 | Book 11 - Part 1, 1 of 2 -- Plasmin Digestion of. 4/74 - 5/74. | |
| 21 | 16 | Book 11 - Part 1, 2 of 2 -- Plasmin Digestion of. 4/74 - 5/74. | |
| 21 | 17 | Book 11 - Part 2, 1 of 2 -- Plasmin Digestion of H0 90' 21hr. 4/74 - 5/74. | |
| 21 | 18 | Book 11 - Part 2, 2 of 2 -- Plasmin Digestion of H0 90' 21 hr. 4/74 - 5/74. | |
| 21 | 19 | Book 12 - Part 1 -- HD, CNBr G-100 Purification, Enzyme Digestions. 1/75 - 3/75. | |
| 22 | 1 | Book 12 - Part 2. 01/75 - 03/75. | |
| 22 | 2 | Book 13. Separation of D and E on Biogel A 1.5 M, 4 m Guanidine, 02/74 - 07/74. | |
| 22 | 3 | Book 14. HDII: Separation, R and A, Purification, Enzyme Digestion, 08/74 - 12/74. | |
| 22 | 4 | Book 15 - Part 1. HE R and A -- G-25, Paper El, 07/74. | |
| 22 | 5 | Book 15 - Part 2. 07/74. | |
| 22 | 6 | Book 16 - Part 1. Gel Filtration of D and E, HE CNBr -- G-50, Paper El of Peptides, 05/74- 07/74. | |
| 22 | 7 | Book 16 - Part 2. 05/74 - 07/74. | |
| 22 | 8 | Book 17 - Part 1. HE CNBr (S-S) in E, Purification of CNBr Peptides, 08/74 - 01/75. | |
| 22 | 9 | Book 17 - Part 2. 08/74 - 01/75. | |
| 22 | 10 | Book 18 - Part 1. FIIB-2, FIIB-3, FIIB-4, FIIB-5, D-D3, 02/74 - 02/75. | |
| 22 | 11 | Book 18 - Part 2. 02/74 - 01/75. | |
| 22 | 12 | Book 19 - Part 1. CNBr Digestion of H Fibrin, 10/74 - 02/75. | |
| 22 | 13 | Book 19 - Part 2. 10/74 - 02/75. | |
| 22 | 14 | Book 20 - Part 1. TATG of H Fragments D, E, 02/74. | |
| 22 | 15 | Book 20 - Part 2. TATG of H Fragment D, E, 02/74. | |
| 22 | 16 | Book 21. H0 CNBR, H Gamma CNBr, 02/74 - 5/74. | |
| 22 | 17 | Book 22. H 14C GEE Labelled Gamma Chain, Fragments from Plasmin Digestion of H GEE Fibrins, 02/74 - 05/74. | |
| 22 | 18 | Book 23. TATG of H Fragment D, E (see envelope), 05/74 - 8/74. | |
| 22 | 19 | Book 24. Human Gamma G-50, Purfication of Pools 4.5 and 6, Enzyme Digestion of Cyanogen Bromide Pools, 12/74 - 02/75. | |
| 23 | 1 | Book 25 - Part 1. Beta chain CNBr, G-100 10% HAc Purfication, 08/74 - 12/75. | |
| 23 | 2 | Book 25 - Part 2. 08/75 - 12/75. | |
| 23 | 3 | Book 26 - Part 1. Separation of H Gamma, Beta Chains CM52, Beta Chain CNBr Peptides AAA, 03/74. | |
| 23 | 4 | Book 26 - Part 2. 03/74. | |
| 23 | 5 | Book 27. Bov Gamma CNBr A,B,C, AAA (Bovine). | |
| 23 | 6 | Book 28. Oxidized Bov Gamma Chain and Its CNBr Fragments, Separation Biogel 1.5 Purification by Paper El, 01/74 - 02/74. | |
| 23 | 7 | Book 29. H Fragment A from Plasmin, digest 10', 90', 15 hrs, Enzyme Digestion, 05/72. | |
| 23 | 8 | Book A - Part 1. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platelet "X", 12/01/72 - 10/31/73. | |
| 23 | 9 | Book A - Part 2. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X", 12/01/72 - 10/31/73. | |
| 23 | 10 | Book A - Part 3. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X". | |
| 23 | 11 | Book A - Part 4. HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for TATG; P - Ser; Pepsin; Platetet "X", 12/01/72 - 10/31/73. | |
| 23 | 12 | Book B - Part 1. Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels); Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *; Separation of A and B Chains of F XIII *, 09/01/72 - 09/30/73. | |
| 23 | 13 | Book B - Part 2. Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels); Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *; Separation of A and B Chains of F XIII *, 09/01/72 - 09/30/73. | |
| 23 | 14 | Book C - Part 1. TATG: H, B FXIII, FXIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72 - 11/31/73. | |
| 23 | 15 | Book C - Part 2. TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII; Seattle F XIII *, 11/01/72 - 11/31/73. | |
| 23 | 16 | Book C - Part 3. TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72 - 11/31/73. | |
| 23 | 17 | Book D - Part 1. Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th, 10/01/72 - 10/31/73. | |
| 23 | 18 | Book D - Part 2. Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th, 10/01/72 - 10/31/73. | |
| 23 | 19 | Book E. Bov Gamma ( plasmin, trypsin SDS gels ); GEE B Gamma - Plasmin; B 0 plasmin, 08/01/72 - 03/31/73. |
Miscellaneous Researchers
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| 24 | 1 | Friezner, S - Book 1 - Part 1. H Gamma CNI Trypsin, 06/75. | |
| 24 | 2 | Friezner, S - Book 1 - Part 2. 06/75. | |
| 24 | 3 | Lin, Y - Book 1. H 14C GEE Alpha Electrophoresis; T-boc Reactions T-boc-EA, 02/01/71 - 05/31/71. | |
| 24 | 4 | Lin, Y - Book 2 - Part 1. Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains, 02/01/71 - 05/31/74. | |
| 24 | 5 | Lin, Y - Book 2 - Part 2. Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains, 02/01/71 - 05/31/74. | |
| 24 | 6 | Maffly, B. and Goldbaum, D - HPLC. 04/76 - 01/78. |
Electric Birefringence
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| 24 | 7 | Cottrell, B. - Book 1 - Part 1, 8/73 - 11/73. | |
| 24 | 8 | Cottrell, B. - Book 1 - Part 2, 8/73 - 11/73. | |
| 24 | 9 | Cottrell, B. - Book 2 - Part 1, 1973. Photographs, computer programs and basic user manual. | |
| 24 | 10 | Cottrell, B. - Book 2 - Part 2, 4/74 - 6/74. | |
| 24 | 11 | Cottrell, B. - Book 2 - Part 3, 4/74 - 6/74. | |
| 24 | 12 | Cottrell, B. - Book 3 - Part 1, 6/74 - 7/74. | |
| 24 | 13 | Cottrell, B. - Book 3 - Part 2, 6/74 - 7/74. | |
| 24 | 14 | Cottrell, B. - Book 4 - Part 1, 8/74 - 3/75. | |
| 24 | 15 | Cottrell, B. - Book 4 - Part 2, 8/74 - 7/75. | |
| 24 | 16 | Cottrell, B. - Book 5 - Part 1, 3/75 - 5/75. | |
| 24 | 17 | Cottrell, B. - Book 5 - Part 2, 3/75 - 5/75. | |
| 24 | 18 | Cottrell, B. - Computer Graphs B/C. | |
| 25 | 1 | Cottrell, B. - Computation Book, Oct 22, 1973. | |
| 25 | 2 | Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 1. | |
| 25 | 3 | Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 2. | |
| 25 | 4 | Cottrell, B. - Data Output. Elbra - Part 1. | |
| 25 | 5 | Cottrell, B. - Data Output. Elbra - Part 2. | |
| 25 | 6 | Cottrell, B. - Log Book. Photographs. | |
| 25 | 7 | Cottrell, B. - Log Book. Photographs - Enlarged (part II). | |
| 25 | 8 | Troll, M. - Book 1. System logic of the laser birefringence machine, reprints, technical information, equipment specifications (part 1). | |
| 25 | 9 | Troll, M. - Book 1. System logic of laser birefringence machine, reprints, technical information, equipment specifications (part II). |
Peptide Synthesis
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| 25 | 10 | BM BIOCHEMICA - Vol. 2 Number 3, 1985. | |
| 25 | 11 | Book 9. | |
| 25 | 12 | Book 10. | |
| 25 | 13 | Computation Notebook #1. | |
| 25 | 14 | Computation Notebook #2. | |
| 25 | 15 | Computation Notebook #3. | |
| 25 | 16 | Computation Notebook #4. | |
| 25 | 17 | Computation Notebook #5. | |
| 25 | 18 | Computation Notebook #6. | |
| 26 | 1 | Computation Notebook #7. | |
| 26 | 2 | Computation Notebook #8. | |
| 26 | 3 | Computation Notebook #9. | |
| 26 | 4 | Computation Notebook #10. | |
| 26 | 5 | Computation Notebook #11. | |
| 26 | 6 | Computation Notebook #12. | |
| 26 | 7 | Deoxynucleotide Synthesis, Phosphonylations, Monomer Synthesis, Dimer Synthesis. | |
| 26 | 8 | DNA Sequencing 3:728-3D, 1985. | |
| 26 | 9 | D.T. Peptide Synthesis. | |
| 26 | 10 | Evans, Barbara - Untitled notebook. | |
| 26 | 11 | Gel Sequencing. | |
| 26 | 12 | Helices. | |
| 26 | 13 | Lab Reports. | |
| 26 | 14 | Lamprey Transferrin (Part I). Barbara Evans, Summer 1983. | |
| 26 | 15 | Lamprey Transferrin (Part II). Barbara Evans, Summer 1983. | |
| 26 | 16 | Lamprey Transferrin: DNA Sequencing 463-1B, 1985 - 1986. Barbara Evans. | |
| 26 | 17 | Lamprey Transferrin: Plasmid Preps, 1984. Barbara Evans. | |
| 26 | 18 | Lamprey Transferrin: Sequence 1 Molecular Biology, 1984 - 1985. Barbara Evans. | |
| 26 | 19 | Lamprey Transferrin: Sequencing 2: 613-3B, 463-1B, 1985. Barbara Evans. | |
| 26 | 20 | Nucleotide Synthesis - Ologonucleotide Probes, 1982. Barbara Evans. | |
| 26 | 21 | Peptide Synthesis - James Kobut/R. Doolittle. | |
| 27 | 1 | Pontes, Manuel. | |
| 27 | 2 | Pontes, Manuel. | |
| 27 | 3 | RD and MTNICB0. |
Analysis Charts
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| 27 | 4 | 2 VB Sequence. | |
| 27 | 5 | AAA of Gamma Chains Trp Det. | |
| 27 | 6 | Bead Data SP Digest of PLI (Part I). | |
| 27 | 7 | Bead Data SP Digest of PLI (Part II). | |
| 27 | 8 | Bead Data SP Digest PLI (Part II continues). | |
| 27 | 9 | Bov Gamma Chains, 1973. | |
| 27 | 10 | Bov Gamma chains II. | |
| 27 | 11 | CN I, PL I, SP I, PL II, Tryptic Data, Chymo, SP Peptides, 1979. | |
| 27 | 12 | CN - V B, C, and D. B Gamma CN from pH2, SC - pH 9.5, CN5B - pH 9.5, SD - pH 4.5. | |
| 27 | 13 | CNBr, Cyanogen Bremide G-75 Runs. | |
| 27 | 14 | Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part I). | |
| 27 | 15 | Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part II). | |
| 27 | 16 | D1 Binder D1 Non Binder 12/80 R and A Trypsin. | |
| 28 | 1 | D2 Alpha - Trypsin. | |
| 28 | 2 | D2 Rho Glass Beads. | |
| 28 | 3 | H Alpha 2, CNI, TFA, CN IB, Trypsin, part 1. | |
| 28 | 4 | H Alpha 2, CNI, TFA, CN IB, Trypsin, part 2. | |
| 28 | 5 | H2 CN I PL I Se #2, S rho I TR - G-25, part 1. | |
| 28 | 6 | H2 CN I PL I Se #2, S rho I TR - G-25, part 2. | |
| 28 | 7 | H Alpha CNI CNBr = 70% TFA, H0CNI Pl I Glu, Pl 2 Glu. | |
| 28 | 8 | H and CNI PL 3. | |
| 28 | 9 | H Alpha 2, 1977. | |
| 28 | 10 | H Alpha 2, 1977. | |
| 28 | 11 | H Alpha CN, 1976. | |
| 28 | 12 | H Alpha CN III, 1976. | |
| 28 | 13 | H Alpha CN III, 1978. | |
| 28 | 14 | H Alpha CN III, 1978. | |
| 28 | 15 | H Alpha CN IV, 1975. | |
| 28 | 16 | H Alpha CN IV , 1975. | |
| 28 | 17 | H Alpha CN4A. | |
| 28 | 18 | H Alpha CN IV B. | |
| 29 | 1 | H Alpha CN31. | |
| 29 | 2 | H Alpha CN5B. | |
| 29 | 3 | H Gamma Chain CN IV, 1975. | |
| 29 | 4 | H Alpha CN CN -VII, 1977 (1 of 2). | |
| 29 | 5 | H Gamma Chain CN V-VII, 1977 (2 of 2). | |
| 29 | 6 | HD R and A BC 1980 D1 Gamma D3 Gamma Trypsin. | |
| 29 | 7 | H Gamma chain, 1971. | |
| 29 | 8 | H Gamma chain, 1972. | |
| 29 | 9 | H Gamma chain, 1972. | |
| 29 | 10 | Gamma Chains Preps CN III, CNIV. | |
| 29 | 11 | HXLF CEE 7177 CN I Tal 23. | |
| 29 | 12 | Machine H Alpha CN III "G7B", 1976. | |
| 29 | 13 | Mellitin Sequence Run, 07/18/80. | |
| 29 | 14 | Sequence Guide 1969-70. | |
| 29 | 15 | Sequenator log sheet. | |
| 30 | 1 | SP Digest #4 PLI, TR Digest Over G.50, part 1. | |
| 30 | 2 | SP Digest #4 PLI, TR Digest Over G.50, part 2. | |
| 30 | 3 | SP Digest CNI #6, SP Digest CNI #9 (8/79), SP Digest CNI #11 (4/79). | |
| 30 | 4 | SP of PLI #3, SP of PLI #5. 11/15/78. | |
| 30 | 5 | Trypsin Digest. | |
| 30 | 6 | Untitled. | |
| 30 | 7 | Untitled. | |
| 30 | 8 | Untitled. | |
| 30 | 9 | Untitled. | |
| 30 | 10 | Untitled. | |
| 30 | 11 | Untitled. | |
| 30 | 12 | Untitled. | |
| 30 | 13 | Untitled. | |
| 30 | 14 | Untitled. | |
| 30 | 15 | Untitled. |
High Performance Liquid Chromotography Charts
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| 31 | 1 | Untitled. | |
| 31 | 2 | Untitled. | |
| 31 | 3 | Untitled. | |
| 31 | 4 | Acylase, N-Ac-Ab-C-Sar. | |
| 31 | 5 | CMC. | |
| 31 | 6 | Electrofocusing. | |
| 31 | 7 | Funatsu Peptide. | |
| 31 | 8 | HPLC Anion Exchange. | |
| 31 | 9 | HPLC, DMG, PTH, STDS. | |
| 31 | 10 | HPLC JD. | |
| 31 | 11 | HPLC Reverse Phase, March-April 1976. |
AMINO ACID SEQUENCER
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| 31 | 12 | AB-000-01 | Amino Acid Sequencer. |
Finding aid generated: 2005-10-28